Journal of General Virology

Fig. 1. Plasmid map of the expression vector used to produce the Flag–E2 fusion protein. The vector was transfected into COS-7 cells and cells were harvested 3 days later. The E2–Flag fusion protein was purified by using anti-Flag affinity columns (Anti-Flag M2 affinity gel; Eastman Kodak) and inoculated into two rabbits. Sera were screened for reactivity to the inoculated protein and serum from one of the rabbits (day 85) was used as a source of anti-E2 antibody.

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