Journal of General Virology

Fig. 3. Negative-strand-specific RT–PCR of cell cultures. Cells were infected with GBV-C/HGV-containing serum and harvested at different time-points post-inoculation. RNA was extracted, cDNA was synthesized by using the negative-strand-specific primer (GBO), nested PCR was performed and the products were visualized in ethidium bromide-stained agarose gels. The PCR product has a predicted size of 207 bp.

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