Journal of General Virology

Fig. 1. Surface or intracellular immunostaining of transiently transfected ScN2a cells expressing the following constructs: (a) MHM2PrP surface staining, (b) MHM2PrP intracellular staining, (c) MHM2PrP(N180Q,N196Q) surface staining, (d) MHM2PrP(N180Q,N196Q) intracellular staining, (e) MHM2PrP(T182A,T198A) surface staining, (f) MHM2PrP(T182A,T198A) intracellular staining. Whereas both MHM2PrP and MHM2PrP(N180Q,N196Q) are stained on the cell surface (a, c) as well as in intracellular compartments (b, d), MHM2PrP(T182A,T198A) is positive for intracellular staining (f) but not for cell surface staining (e), suggesting that the latter construct is either retained in intracellular compartments or rapidly degraded on the cell surface.

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