Korth et al. - Fig. 2

Journal of General Virology

Fig. 2

Fig. 2. Western blot of the expression and conversion of unglycosylated mutated PrP in ScN2a cells. Transiently transfected ScN2a cells express MHM2PrP(N180Q,N196Q) with an expected gel mobility of 24 kDa, which is converted into MHM2PrP^Sc(N180Q,N196Q) (gel mobility of 16 kDa), as is MHM2PrP. MHM2PrP(T182A,T198A) is not as efficiently converted. Addition of mutation Q218K to MHM2PrP(N180Q,N196Q) abolishes conversion, as it does in MHM2PrP.

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