Journal of General Virology

Fig. 1. (A) Western blot analysis of PrP^C in the spinal cords (sc) and sciatic nerves (scn) of C57Bl/6 (Bl/6) and tga20 mice. The amount of total protein loaded is indicated for each lane. (B) PrP^C content in sciatic nerves was quantified by direct recording of Western blot chemiluminescence using the Kodak 1D image analysis system. A standard curve was produced by plotting the signal intensity produced by 5–40 µg of tga20 sciatic nerve homogenate. The arrow indicates that the PrP^C content of 40 µg wild-type sciatic nerve corresponds to 8 µg tga20 sciatic nerve. (C) Western blot analysis of PrP^C of spleens and lymph nodes (LN) of C57Bl/6 (Bl/6) and tga20 mice. The amount of total protein loaded in each lane was 120 µg.

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