Journal of General Virology

Fig. 3. Western blot detection of PrP^C and PrP^Sc. Upper blot, extracts of spinal cords; lower blot, extracts of sciatic nerves. Both the side of i.n. prion injection (ipsi) and the contralateral (contra) side were examined. Genotypes of mice, route of inoculation and proteinase K digest are indicated above each lane. As a control, we analysed terminally sick intracerebrally inoculated CD1 mice (CD1), mock injected wild-type mice (Bl/6) and PrP knockout mice (PrP^o/o). The amount of total protein loaded in each lane was 50 µg except for the Bl/6 sciatic nerve, where it was 25 µg. While PrP^Sc was readily detectable in the spinal cords of all examined mice, no proteinase K-resistant PrP could be detected in any of the investigated sciatic nerve samples.

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