Journal of General Virology

Fig. 6. Western blot analysis of spleens. Immunodetection with antibody 1B3 (Farquhar et al., 1996) revealed the presence of proteinase K-resistant PrP^Sc in spleens of terminally sick wild-type (n=2) and tga20 (n=2) mice after i.n. and f.p. injection of scrapie prions. No or very little proteinase K-resistant PrP^Sc could be detected in the i.p. or f.p. injected tga20 mice. Indicated above each lane is the genotype of the mouse, the route of inoculation of infectivity, and proteinase K digest. Control experiments were performed with terminally sick intracerebrally inoculated CD1 mice (CD1); mock injected wild-type mice (Bl/6) and PrP knockout mice (PrP^0/0). The amount of total protein loaded in each lane was 80 µg.

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