Journal of General Virology

Cell-free synthesis of poliovirus: 14S subunits are the key intermediates in the encapsidation of poliovirus RNA

Yvan Verlinden,¹ Andrea Cuconati,² Eckard Wimmer² and Bart Rombaut¹

¹ Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium
² Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794, USA

In a cell-free system of uninfected HeLa cells, programmed with poliovirus RNA, extraneous radiolabelled 14S subunits assembled with endogenous 14S subunits and interacted with newly synthesized RNA to form virions (160S). This result suggests that 14S subunits are the key intermediates in the encapsidation of poliovirus RNA.

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© 2000 SGM

This article is now available in the November 2000 print issue of JGV (vol. 81, 2751–2754). The complete issue of the journal may be seen in electronic form on JGV Online.