Journal of General Virology

Fig. 3. Detection of MHV-68 in mice by nested PCR. DNA was extracted from the organs of mice and analysed for the presence of MHV-68 by nested PCR using primers specific for the gp150 gene (Stewart et al., 1998). Products were analysed on 2 % agarose gels containing ethidium bromide and visualized using a transilluminator. Molecular size determinations were made relative to a 1 kb DNA ladder (Mr), and the sizes of the pertinent bands are shown at the left. In all panels, DNA from the MHV-68-positive cell line S11 was used as a positive control (+VE) and produced the expected product of 368 bp. DNA from the MHV-68-negative tumour line S31 was used as a negative control (-VE). (A) Analysis of the brains of five CBA mice that were infected by implantation with infected MGC7 cells 12 months previously. (B) Analysis of spleens, blood and brains of five 129 mice that had been infected intranasally 12 months previously with MHV-68.

Back to article

0001-7291 © 2000 SGM