Journal of General Virology

Fig. 2. Binding of StpB to c-Src. AU1 epitope-tagged StpB (StpB), mutants AU–StpB Y₆₂F (Y62F) and AU–StpB Y₁₁₈F (Y118F) as well as c-Src (Src) were expressed in COS cells either alone or in the combinations indicated. Src complexes were immunoprecipitated from cell lysates with SRC2 antibody (IP SRC2). Precipitation with protein A–Sepharose alone (IP no ab) served as a negative control. AU1-specific immunoblots (IB anti-AU1) were used to detect StpB proteins in precipitates (upper panel) and cell lysates (lower panel). Expression of c-Src was confirmed by an immunoblot with cell lysates and MAb EC10 (IB EC10; middle panel). Sizes of molecular mass markers are given in kDa. *, Background band present in all samples containing protein A–Sepharose.

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