Journal of General Virology

Fig. 2. (A) ORFs predicted in intron III-spliced mRNAs. The ORFs are designated according to their predicted molecular masses (p190, 190 kDa etc.). The end of each arrow indicates the position of the downstream GE signal closest to the stop codon of the corresponding ORF. (B) p165 protein accumulates in the ER/Golgi apparatus. cDNAs containing p190 and p165 ORFs were tagged at the C terminus with a c-Myc epitope and cloned into the pol II expression vector pCAGGS. Cos-7 cells were transfected and fixed 36 h later and analysed by indirect immunofluorescence with a mouse monoclonal antibody to c-Myc, followed by an FITC-labelled anti-mouse IgG.

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