Journal of General Virology

Fig. 5. The amount of LDH retained in PS cells was used to estimate virus cytotoxicity. PS cells still attached to the wells of 24-well plates after 72–80 h of virus infection were thoroughly washed to remove cell debris, frozen at –70 °C and lysed to release LDH. The substrate for LDH was added to each well and the absorbance was measured at 492 nm after 30 min of incubation at room temperature. The result for each virus is presented as a histogram with five repeated tests.

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