Journal of General Virology

Fig. 4. Analysis of viral RNA accumulation in the fourth (from top) leaf from transgenic Rainbow (RB) and non-transgenic Sunrise (SR) plants. Papaya plants were inoculated with HA, a Hawaii PRSV strain; YK, a Taiwan PRSV strain, or the various recombinant viruses. The sample labelled YK-AS1 was taken from the fourth (from top) leaf with very few flecks. Sample YK-AS2 was taken from the second (from top) leaf without symptoms. Fifty ng of in vitro transcripts from pT3-HAG was used as a positive control. Mock, Inoculated with buffer. (A) Northern blot analysis of total plant RNA hybridized with a ³²P-labelled, 1.2 kb DNA specific for the 3´ end of PRSV HA, including 200 nt of NIb, the complete CP and 3´ NCR. The positions of markers (in kb) are shown on the left. (B) Ethidium bromide-stained gel.

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