Journal of General Virology

Fig. 6. Infectivity of HSV-1 gC mutant viruses on heparinase-treated GMK-AH1 cells. Viruses were pre-diluted and approximately 200–300 p.f.u. of each mutant was used for comparison of infectivity on untreated and heparinase-treated cells. Monolayers of GMK-AH1 cells in 6-well plates were pre-treated with heparinase (1 unit/ml) for 1 h at 37 °C. Virus was then allowed to adsorb to the cells for 30 min at 37 °C, whereafter the cells were covered with methylcellulose and incubated in 37 °C to allow virus plaque formation. Cells were stained with crystal violet and plaques were counted. The results are expressed as the number of plaques formed on heparinase-treated cells as compared to the control, i.e. the number of plaques formed on monolayers not subjected to pre-treatment with heparinase.

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