Journal of General Virology

Fig. 5. Processing of wild-type and cleavage site mutants of EBOV, species Reston. HeLa cells were infected with a vaccinia virus expressing the T7 polymerase (vTF7-3) and then transfected with the plasmids pGEM-PR8 (wild-type Reston GP, WT) and pGEM-R/R (mutant R/R). At 6 h post-infection, cells were pulse-labelled for 20 min with [³⁵S]cysteine and then chased for 240 min. Immunoprecipitated proteins were separated under reducing conditions on an 8 % polyacrylamide gel. The positions of the non-cleaved precursors (pre-GP_ER and pre-GP) and the cleavage subunit GP₁ are indicated.

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