Journal of General Virology

Fig. 3. Immunoprecipitation (IP) of PrP^C from different tissue extracts by both Pc248 anti-PrP MAb (A, B) and an anti-PrP unrelated control mouse IgG (C) analysed by Western blot (WB) with either MH44 anti-PrP IgG (A, C) or biotinylated 4F2 IgG (B). Tissues were taken from ewe VII. Asterisks indicate the PrP^C doublet detected from skeletal muscle homogenate. The different tissues tested were as follows: brain (Br), tongue (Tg), tonsil (Ts), lung (Lu), heart (H), spleen (Sp), liver (Li), skeletal muscle (SM), reticulum (Re), rumen (Ru), duodenum (Du), jejunum (Je), ileum (IL), mammary glands (Ma) and pancreas (Pa). Lane Br1/4 shows a fourth dilution of the PrP^C fraction obtained from brain to distinguish the three PrP^C glycoforms.

Back to article

0001-7776 © 2001 SGM