Journal of General Virology

Fig. 4. PrP^C distribution in different tissues from ewe VII using 4F2 IgG for immunoprecipitation (IP) followed by Western blot (WB) detection with either biotinylated 4F2 IgG (A) or MH44 anti-PrP PAb IgG (B). The different tissues tested were as follows: brain (Br), tongue (Tg), tonsil (Ts), lung (Lu), heart (H), spleen (Sp), liver (Li), skeletal muscle (SM), reticulum (Re), rumen (Ru), duodenum (Du), jejunum (Je), ileum (IL) and mammary glands (Ma). (C) IP of PrP^C from H, SM and Br samples from ewes III and II, indicating the specific muscle profile of PrP^C. Note the difference in migration between the bi- and monoglycosylated isoforms of brain PrP^C and the PrP^C doublet observed in SM. Bi-, mono- and nonglycosylated (B, M and N) isoforms described in the brain are indicated on the right.

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