JGV Direct - CP gene-mediated resistance to PVA (E. I. Savenkov and J. P. T. Valkonen) ABSTRACT

Journal of General Virology

First posted online 11 June 2001	ARTICLE ABSTRACT
Rec 4 April 2001; Acc 30 May 2001	DOI: 10.1099/vir.0.17781-0

Coat protein gene-mediated resistance to Potato virus A in transgenic plants is suppressed following infection with another potyvirus

E. I. Savenkov and J. P. T. Valkonen

Department of Plant Biology, Genetics Centre, SLU, PO Box 7080, S-750 07 Uppsala, Sweden

High levels of resistance to Potato virus A (PVA, genus Potyvirus), indicated by absence of detectable infection in inoculated leaves, were attained in Nicotiana benthamiana transformed with a construct expressing the PVA 5´-untranslated region fused with the coat protein (CP)-encoding sequence. Low steady-state levels of the transgene transcripts were detected. Resistance was PVA-specific and did not protect the plants against infection with Potato virus Y (PVY, genus Potyvirus). Consequently, the steady-state levels of the CP-transgene mRNA were greatly elevated in the plants infected with PVY, and plants became susceptible to infection with PVA. These data show that virus resistance obtained by expressing regions of a plant virus genome in transgenic plants may be suppressed following infection with another virus that evades the virus-specific resistance.

Click here for full text HTML

JGV Direct table of contents

This article is now available in the September 2001 print issue of JGV (vol. 82, 2275–2278). The complete issue of the journal may be seen in electronic form on JGV Online.