Journal of General Virology

Supplementary data. Immunoblot of purified IMV and EEV using Rb-WR2 Ab. Purified IMV and EEV (10 µg each) were resolved by SDS–PAGE (12 % gel) and transferred to a nitrocellulose membrane. Virus-specific proteins were detected with Rb-WR2 IgG (0.25 µg/ml) and horseradish peroxidase-conjugated goat anti-rabbit IgG (diluted 1/2000, Sigma) using an ECL Western Blotting detection system (Amersham Pharmacia Biotech). Common bands represent the immunodominant IMV and core proteins shared by IMV and EEV. Potential EEV-specific proteins are indicated by arrows.

Back to article

0001-8005 © 2001 SGM