Journal of General Virology

Fig. 1. The location of RSV filaments on the surface of virus-infected cells. (A) Western blot analysis of mock-infected (M) and RSV-infected (I) cells using anti-RSV and mAb19. The positions of the specific RSV proteins are indicated. (B) Localization of RSV filaments on the apical surface of RSV-infected Vero C1008 cells using fluorescence microscopy. Mock (M) and RSV (I)-infected cells were permeabilized and labelled using mAb19 or anti-RSV. The RSV filaments (VF) and an inclusion body (IB) are indicated, as is the magnification of the objective lens used (x20, x40 or x64). (C) Analysis of RSV-infected cells using SEM. The cell surface of mock-infected (M) and RSV-infected (I) cells was examined by SEM. A cluster of viral filaments (VF) is indicated and the arrows show the limits of a single large RSV filament cluster. Magnification x15000. Bar indicates 1 µm.

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