Journal of General Virology

Fig. 5. TEM analysis of RSV-associated cav-1 by immuno-gold labelling with anti-cav-1. (A) Thin sections from RSV-infected cells were labelled using anti-cav-1, the presence of which was observed by the appearance of 10 nm colloidal gold. Plates 1 and 2 are different views of RSV filaments that have been decorated with 10 nm gold particles. Plate 3 shows double immuno-labelling with 20 nm (cav-1) and 10 nm (anti-RSV) colloidal gold. In this view, both a longitudinal and cross-sectioned filament are shown. Plate 4 shows that at the initial stages of RSV assembly, the early budding virus particles can be labelled using anti-cav-1 and decorated with 10 nm gold particles. Furthermore, the gradual acquisition of an electron-dense core in the early budding RSV particles can be seen in plate 3. (B) RSV particles were harvested from the tissue culture medium of virus-infected cells and the presence of cav-1 was detected by immuno-gold labelling. Plate 1 shows a mechanically disrupted RSV particle in which the viral envelope (VE) and RNP are clearly visible. The viral envelope is decorated at four places with 10 nm gold after labelling with anti-cav-1. Plate 2 shows immuno-negative staining of an intact RSV particle by double-labelling using 5 nm (mAb19) and 10 nm (cav-1) colloidal gold particles. The 10 nm gold particles are indicated by asterisks.

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