Computational sequence analysis is no longer, if ever it was, the exclusive preserve of specialist bioinformaticians. BLAST, multiple sequence alignment, and phylogeny inference are as much a part of the core skill-set as PCR and SDS- PAGE and just as reliant on the availability of tried and tested protocols. This book sensibly concentrates almost exclusively on DNA, though the chapter on BLAST includes a very helpful primer on the PAM and BLOSUM protein similarity matrices. Inevitably, the range of tools included is not comprehensive. Whole-genome alignment is shoe-horned into an otherwise excellent chapter on multiple sequence alignment using MAFFT. But, I discovered several new ones that I might use (e.g. DataMonkey). The target audience seems to vary, some chapters assuming familiarity with working in a command-line environment, others restricted to web-based tools. However, I can imagine using chapters of this book in my own teaching of MSc Bioinformatics students or advanced undergraduates.